Frozen In Ice (Ice Breaker Cold Case Romance Book 1)

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Zhong ZP, Rapp JZ, Wainaina JM, Solonenko NE, Maughan H, Carpenter SD, et al. Viral ecogenomics of Arctic cryopeg brine and sea ice. mSystems. 2020;5:e00246–20. Department of Civil, Environmental and Geodetic Engineering, Ohio State University, Columbus, OH, USA

A comprehensive theory of ice friction takes into account all the above-mentioned friction mechanisms. [53] This model allows quantitative estimation of the friction coefficient of ice against various materials as a function of temperature and sliding speed. In typical conditions related to winter sports and tires of a vehicle on ice, melting of a thin ice layer due to the frictional heating is the primary reason for the slipperiness. [ citation needed] The mechanism controlling the frictional properties of ice is still an active area of scientific study. [54] Natural formation Feather ice on the plateau near Alta, Norway. The crystals form at temperatures below −30°C (−22°F). In all generations except Generation I, freeze has a chance to be cured on its own on the frozen Pokémon's turn; it has a 10% chance in Generation II, and 20% chance in all subsequent generations. Consequently, the frozen Pokémon may thaw out on the turn of freezing. Pokémon cannot be frozen in harsh sunlight, but harsh sunlight has no effect on Pokémon that are already frozen. Vikram S, Guerrero LD, Makhalanyane TP, Le PT, Seely M, Cowan DA. Metagenomic analysis provides insights into functional capacity in a hyperarid desert soil niche community. Environ Microbiol. 2016;18(6):1875–88. https://doi.org/10.1111/1462-2920.13088. Total numbers of CBA 18 in each of the artificial ice samples (i.e., Cut1, Wash1, Inner1, Cut2, Wash2, Inner2, and Mix; Fig. 1a) were quantified using the primer set Cbal18M666_05390F (5′-ACGTACAAATAAGGAGAATGGCTT-3′) and Cbal18M666_05390R (5′-AGCGCTAATCCCTGTTGAGA-3′), which specifically targets a 61 bp fragment of an ATP synthase subunit C of CBA 18, with thermocycling: 95°C for 15 min, 45 cycles of 95°C for 15 s, 60°C for 30 s, and 70°C for 25 s. Similarly, total PSA-HP1 numbers of these samples were quantified using strain-designed primer set 10-94a_dF (5′-TCTCTCGTCTTAATGACTTTCATCAT-3′) and 10-94a_dR (5′-TTCTTTCTCAACTTCCTGCTCTAA-3′), with the identical thermocycling conditions, except that 50 cycles of amplifications were conducted. The standard curves of the above two qPCRs were generated with the PCR products from their primer sets and strains, respectively. Tag-encoded amplicon sequencing of the microbial communityIn Toying With Your Motions!, Ash's Dracovish froze Leon's Dragapult with Ice Fang in an attempt to prevent it from using Dragon Tail, but failed to completely freeze over Dragapult's tail, allowing the move to land and free Dragapult from the ice in the process.

Pratama AJ, Bolduc B, Zayed AA, Zhong ZP, Guo J, Vik D, et al. Expanding standards in viromics: in silico evaluation of dsDNA viral genome identification, classification, and auxiliary metabolic gene curation. PeerJ. 2021;9:e11447. https://doi.org/10.7717/peerj.11447. Kalyaanamoorthy S, Minh BQ, Wong TKF, von Haeseler A, Jermiin LS. ModelFinder: fast model selection for accurate phylogenetic estimates. Nat Methods. 2017;14(6):587–9. https://doi.org/10.1038/nmeth.4285. Physical properties The three-dimensional crystal structure of H 2O ice I h (c) is composed of bases of H 2O ice molecules (b) located on lattice points within the two-dimensional hexagonal space lattice (a). [8] [9]Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA McDaniel L, Paul JH. Effect of nutrient addition and environmental factors on prophage induction in natural populations of marine synechococcus species. Appl Environ Microbiol. 2005;71(2):842–50. https://doi.org/10.1128/AEM.71.2.842-850.2005. Castello JD, Rogers SO, Starmer WT, Catranis CM, Ma LJ, Bachand GD, et al. Detection of tomato mosaic tobamovirus RNA in ancient glacial ice. Polar Biol. 1999;22(3):207–12. https://doi.org/10.1007/s003000050411.

In Pokémon Emerald, when the player is inside the Battle Pyramid, the types of Pokémon encountered on each floor follow a set of categories; one of these categories is Pokémon with moves that freeze. No types are immune to freeze, but Pokémon with the Adept, Ice Boost, or Steady Special Traits cannot be frozen, and those with the Reflector Trait will cause the user of the freeze-inducing move to become frozen instead if hit by one.

A great mystery

Roux S, Emerson JB, Eloe-Fadrosh EA, Sullivan MB. Benchmarking viromics: an in silico evaluation of metagenome-enabled estimates of viral community composition and diversity. PeerJ. 2017;5:e3817. https://doi.org/10.7717/peerj.3817.

Zhi-Ping Zhong, Funing Tian, Natalie E. Solonenko, Yueh-Fen Li, Ellen Mosley-Thompson, Virginia I. Rich, Matthew B. Sullivan & Lonnie G. Thompson

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Miteva V. Bacteria in snow and glacier ice. In: Margesin R, Schinner F, Marx J-C, Gerday C, editors. Psychrophiles: from biodiversity to biotechnology. Berlin, Heidelberg: Springer Berlin Heidelberg; 2008. p. 31–50. Loveland-Curtze J, Miteva VI, Brenchley JE. Herminiimonas glaciei sp. nov., a novel ultramicrobacterium from 3042 m deep Greenland glacial ice. Int J Syst Evol Microbiol. 2009;59(6):1272–7. https://doi.org/10.1099/ijs.0.001685-0. In Shunning Spiritomb, Cyrus's Weavile froze Cynthia's Spiritomb. Cynthia tried to heal Spiritomb with an Aspear Berry, but Cyrus prevented this by having Weavile use Embargo. This forced the Sinnoh Champion to recall Spiritomb and send her Milotic out in her place. Hodson A, Anesio A, Ng F, Watson R, Quirk J, Irvine-Fynn T, et al. A glacier respires: quantifying the distribution and respiration CO 2 flux of cryoconite across an entire Arctic supraglacial ecosystem. J Geophys Res Biogeosci. 2007;112:G04S36.